{"id":592,"date":"2023-09-16T22:08:11","date_gmt":"2023-09-16T21:08:11","guid":{"rendered":"https:\/\/microregistrar.com\/?p=592"},"modified":"2023-09-16T22:08:11","modified_gmt":"2023-09-16T21:08:11","slug":"culture-media-and-composition","status":"publish","type":"post","link":"https:\/\/microregistrar.com\/?p=592","title":{"rendered":"Culture media and composition"},"content":{"rendered":"\n

This is a list of some culture media and their composition.<\/p>\n\n\n\n

MEDIA<\/th>SUPPLEMENTS<\/th>TARGET ORGANISMS<\/th><\/tr><\/thead>
ABC plates<\/td>Alpha-galactosidase<\/td>Salmonella sp.<\/td><\/tr>
Alkaline peptone water [pH \u2013 8.6 \u00b1 0.2]<\/td>NaCl, peptone<\/td>Enrichment of Vibrio<\/strong> spp.<\/td><\/tr>
Aztreonam Blood Agar<\/td>Aztreonam, blood agar base<\/td>Gram-positive, especially fastidious gram-positive organisms<\/td><\/tr>
BHI Broths<\/td>Calf brain and beef heart infusion solids, peptone<\/td>Used for a broad range of pathogens \u2013
Fastidious anaerobic organisms, Haemophilus influenza, pathogenic fungi and many other organisms (Streptococcus pneumonia, S aureus,  L monocytogenes, Leishmania promastigotes and C difficile<\/em>.)<\/td><\/tr>
Blood Agar<\/td>tryptic soy agar or Columbia agar base with 5% sheep blood.<\/td>Generic enrichment media.<\/td><\/tr>
Charcoal agar
with

Bordetella Selective Supplement (cephalexin)<\/td>		\u2018Lab-Lemco\u2019 powder,
Charcoal agar,
Nicotinic acid,
Starch & peptone.

with cephalexin<\/td>		Bordetella pertussis<\/em> and Bordetella parapertussis<\/em> 

It can be used for H influenzae, if without cephalexin<\/td><\/tr>
Bordet-Gengue<\/td>meat peptone, Caseine peptone, potato infusion, sheep blood<\/td>Non-selective media<\/td><\/tr>
CAA<\/td>Cefalexin atreonam arabinose agar<\/td>Differentiates Enterococcus sp<\/td><\/tr>
Camp plates<\/td>Vancomycin, Polymixin, Trimethoprim<\/td>Campylobacter sp.<\/td><\/tr>
CAN ID<\/td>Vancomycin, Aztrenam, Glucosaminide, Phosphate<\/td>Candida albicans, Candida tropicalis<\/td><\/tr>
Cepacia plates<\/td>Gentamicin and Polymixin B<\/td>Burkholderia cepacia<\/td><\/tr>
Chocolate Agar<\/td>Haemolysed Blood<\/td>Haemophilus species, Neisseria species<\/td><\/tr>
Chocolate Bacitracin Agar<\/td>Bacitracin<\/td>Inhibits Gram-positive growth<\/td><\/tr>
CLED plates<\/td>Cysteine Lactose Electrolyte Deficient<\/td>Differentiation between coliforms<\/td><\/tr>
FAA plates<\/td>Fastidious anaerobe agar, Nalidixic acid<\/td>Anaerobes<\/td><\/tr>
HDA Agar<\/td>Cefoxitin & Cefpodoxime<\/td>ESBLs and MRSA<\/td><\/tr>
IST Agar<\/td>Iso sensitivity test agar<\/td>Sensitivity testing<\/td><\/tr>
Legionella<\/td>Vancomycin hydrochloride, colistin, amphotericin B<\/td>Legionella sp<\/td><\/tr>
Listeria plates<\/td>Aesculin, ferric ammonium citrate, lithium chloride<\/td>Listeria sp<\/td><\/tr>
MTS<\/td>Mannitol trihalose salt<\/td>Differentiate non-fermenters (s.epi) from other CNS<\/td><\/tr>
Mueller Hinton Agar<\/td>Beef, dehydrated infusion from casein hydrolysate, starch<\/td>Detection of growth requirements of Haemophilus sp<\/td><\/tr>
NAD Sensitivity Plates<\/td>Nicotinamide Adenine Dinucleotide<\/td>Sensitivity testing of Haemophilus sp<\/td><\/tr>
Nagler Plate<\/td>Lechinase & egg yolk.<\/td>Clostridium perfringens<\/td><\/tr>
Nali Blood Agar<\/td>Nalidixic acid<\/td>fastidious organisms<\/td><\/tr>
Neomycin Blood Agar<\/td>Neomycin<\/td>Clostridium species<\/td><\/tr>
Nutrient Agar<\/td>Yeast extract, peptone, sodium chloride<\/td>Non-fastidious organisms, e.g. Staphylococcus sp<\/td><\/tr>
NYC<\/td>Robertson cooked meat broth<\/td>Neisseria species<\/td><\/tr>
Robertsons cooked meat broth<\/td>Cooked meat granules, brain heart infusion<\/td>Enrichment broth for most organisms, including anaerobic<\/td><\/tr>
SAB<\/td>Sabouraud Dextrose<\/td>Fungi (yeasts and moulds)<\/td><\/tr>
Selenite<\/td>Peptone, lactose, sodium biselenite<\/td>Salmonella sp<\/td><\/tr>
Sorbitol MacConkey Agar<\/td>Sorbitol, bile salts<\/td>E.coli O157<\/td><\/tr>
TCBS<\/td>Thiosulfate Citrate Bile salts Sucrose<\/td>Vibrio sp<\/td><\/tr>
Tinsdale Agar<\/td>Tellurite<\/td>Corynebacterium diphtheriae<\/td><\/tr>
Uriselect plates<\/td>\u00df-Glucosidase, \u00df-galactosidase, Tryptophan deaminase<\/td>Chromogenic agar to aid identification of organisms<\/td><\/tr>
XLD<\/td>Xylose, Lactose, Sodium desoxycholate<\/td>Xylose non-fermentors<\/td><\/tr>
Yersinia Agar<\/td>Cefsulodin, Irgasan, Novobiocin<\/td>Yersinia sp<\/td><\/tr><\/tbody><\/table><\/figure>\n","protected":false},"excerpt":{"rendered":"

This is a list of some culture media and their composition. MEDIA SUPPLEMENTS TARGET ORGANISMS ABC plates Alpha-galactosidase Salmonella sp. […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-4)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ngg_post_thumbnail":0,"footnotes":""},"categories":[60],"tags":[176,64],"class_list":["post-592","post","type-post","status-publish","format-standard","hentry","category-laboratory","tag-culture-media","tag-laboratory"],"_links":{"self":[{"href":"https:\/\/microregistrar.com\/index.php?rest_route=\/wp\/v2\/posts\/592","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/microregistrar.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/microregistrar.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/microregistrar.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/microregistrar.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=592"}],"version-history":[{"count":1,"href":"https:\/\/microregistrar.com\/index.php?rest_route=\/wp\/v2\/posts\/592\/revisions"}],"predecessor-version":[{"id":593,"href":"https:\/\/microregistrar.com\/index.php?rest_route=\/wp\/v2\/posts\/592\/revisions\/593"}],"wp:attachment":[{"href":"https:\/\/microregistrar.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=592"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/microregistrar.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=592"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/microregistrar.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=592"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}